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affinitypurified rabbit anti phospho stat6 y641 antibody  (R&D Systems)


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    R&D Systems affinitypurified rabbit anti phospho stat6 y641 antibody
    Fig. 10. Western blot analysis of signal transducer and activator of transcription 6 <t>(STAT6)</t> (a) and the phosphorylated, active form of STAT6 (p-STAT6) (b) in the cell lysates of normal human bronchial epithelial cells incubated with IL-13 (10 ng/mL) or IL-4 (1 ng/mL) for 14 days. Lanes show the data from samples treated with either the vehicle of IL-13 or IL-4 (PBS) (lane 1), IL-13 (10 ng/mL) plus the vehicle of antibody (PBS) (lane 2), IL-13 plus anti-IL-13Ra1 antibody (10 mg/mL) (lane 3), IL-13 plus recombinant human soluble IL-13Ra2/Fc (rsIL-13Ra2, 4 ng/mL) (lane 4), IL-4 (1 ng/mL) plus the vehicle of antibody (PBS) (lane 5) or with IL-4 (1 ng/mL) plus rsIL-13Ra2 (4 ng/mL) (lane 6). The arrows show STAT6 (a) and p-STAT6 (b) (100120 kDa). Data are representative of three differ- ent experiments.
    Affinitypurified Rabbit Anti Phospho Stat6 Y641 Antibody, supplied by R&D Systems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/affinitypurified+rabbit+anti+phospho+stat6+y641+antibody/Phospho-STAT6+(Y641)+PE-conjugated+Antibody/pm18028464-103-36-41
    Average 90 stars, based on 1 article reviews
    affinitypurified rabbit anti phospho stat6 y641 antibody - by Bioz Stars, 2026-09
    90/100 stars

    Images

    1) Product Images from "Modulation of mucus production by interleukin-13 receptor alpha2 in the human airway epithelium."

    Article Title: Modulation of mucus production by interleukin-13 receptor alpha2 in the human airway epithelium.

    Journal: Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology

    doi: 10.1111/j.1365-2222.2007.02871.x

    Fig. 10. Western blot analysis of signal transducer and activator of transcription 6 (STAT6) (a) and the phosphorylated, active form of STAT6 (p-STAT6) (b) in the cell lysates of normal human bronchial epithelial cells incubated with IL-13 (10 ng/mL) or IL-4 (1 ng/mL) for 14 days. Lanes show the data from samples treated with either the vehicle of IL-13 or IL-4 (PBS) (lane 1), IL-13 (10 ng/mL) plus the vehicle of antibody (PBS) (lane 2), IL-13 plus anti-IL-13Ra1 antibody (10 mg/mL) (lane 3), IL-13 plus recombinant human soluble IL-13Ra2/Fc (rsIL-13Ra2, 4 ng/mL) (lane 4), IL-4 (1 ng/mL) plus the vehicle of antibody (PBS) (lane 5) or with IL-4 (1 ng/mL) plus rsIL-13Ra2 (4 ng/mL) (lane 6). The arrows show STAT6 (a) and p-STAT6 (b) (100120 kDa). Data are representative of three differ- ent experiments.
    Figure Legend Snippet: Fig. 10. Western blot analysis of signal transducer and activator of transcription 6 (STAT6) (a) and the phosphorylated, active form of STAT6 (p-STAT6) (b) in the cell lysates of normal human bronchial epithelial cells incubated with IL-13 (10 ng/mL) or IL-4 (1 ng/mL) for 14 days. Lanes show the data from samples treated with either the vehicle of IL-13 or IL-4 (PBS) (lane 1), IL-13 (10 ng/mL) plus the vehicle of antibody (PBS) (lane 2), IL-13 plus anti-IL-13Ra1 antibody (10 mg/mL) (lane 3), IL-13 plus recombinant human soluble IL-13Ra2/Fc (rsIL-13Ra2, 4 ng/mL) (lane 4), IL-4 (1 ng/mL) plus the vehicle of antibody (PBS) (lane 5) or with IL-4 (1 ng/mL) plus rsIL-13Ra2 (4 ng/mL) (lane 6). The arrows show STAT6 (a) and p-STAT6 (b) (100120 kDa). Data are representative of three differ- ent experiments.

    Techniques Used: Western Blot, Incubation, Recombinant

    Related Articles

    Saline:

    Article Title: Modulation of mucus production by interleukin-13 receptor alpha2 in the human airway epithelium.
    Article Snippet: The spots were developed with 0.02% 3, 30 diaminobenzidine tetrahydrochloride (Wako) containing 4.5 10 3 % hydrogen peroxide. .. For STAT6 and the p-STAT6, the membranes were then blocked with 5% skimmed milk in Tris-buffered saline containing 0.1% Tween 20 and incubated with either a monoclonal anti-human/ mouse STAT6 antibody (R&D Systems Inc.) or an affinitypurified rabbit anti-phospho-STAT6 (Y641) antibody (R&D Systems Inc.). .. The membranes were then incubated with HRP-conjugated goat anti-mouse IgG (R&D Systems Inc.) and detected with WesternGlo Chemiluminescent Detection Reagent (R&D Systems Inc.).

    Incubation:

    Article Title: Modulation of mucus production by interleukin-13 receptor alpha2 in the human airway epithelium.
    Article Snippet: The spots were developed with 0.02% 3, 30 diaminobenzidine tetrahydrochloride (Wako) containing 4.5 10 3 % hydrogen peroxide. .. For STAT6 and the p-STAT6, the membranes were then blocked with 5% skimmed milk in Tris-buffered saline containing 0.1% Tween 20 and incubated with either a monoclonal anti-human/ mouse STAT6 antibody (R&D Systems Inc.) or an affinitypurified rabbit anti-phospho-STAT6 (Y641) antibody (R&D Systems Inc.). .. The membranes were then incubated with HRP-conjugated goat anti-mouse IgG (R&D Systems Inc.) and detected with WesternGlo Chemiluminescent Detection Reagent (R&D Systems Inc.).



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    affinitypurified rabbit anti phospho stat6 y641 antibody  (R&D Systems)
    90
    R&D Systems affinitypurified rabbit anti phospho stat6 y641 antibody
    Fig. 10. Western blot analysis of signal transducer and activator of transcription 6 <t>(STAT6)</t> (a) and the phosphorylated, active form of STAT6 (p-STAT6) (b) in the cell lysates of normal human bronchial epithelial cells incubated with IL-13 (10 ng/mL) or IL-4 (1 ng/mL) for 14 days. Lanes show the data from samples treated with either the vehicle of IL-13 or IL-4 (PBS) (lane 1), IL-13 (10 ng/mL) plus the vehicle of antibody (PBS) (lane 2), IL-13 plus anti-IL-13Ra1 antibody (10 mg/mL) (lane 3), IL-13 plus recombinant human soluble IL-13Ra2/Fc (rsIL-13Ra2, 4 ng/mL) (lane 4), IL-4 (1 ng/mL) plus the vehicle of antibody (PBS) (lane 5) or with IL-4 (1 ng/mL) plus rsIL-13Ra2 (4 ng/mL) (lane 6). The arrows show STAT6 (a) and p-STAT6 (b) (100120 kDa). Data are representative of three differ- ent experiments.
    Affinitypurified Rabbit Anti Phospho Stat6 Y641 Antibody, supplied by R&D Systems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/affinitypurified+rabbit+anti+phospho+stat6+y641+antibody/Phospho-STAT6+(Y641)+PE-conjugated+Antibody/pm18028464-103-36-41
    Average 90 stars, based on 1 article reviews
    affinitypurified rabbit anti phospho stat6 y641 antibody - by Bioz Stars, 2026-09
    90/100 stars
    		  Buy from Supplier

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    Fig. 10. Western blot analysis of signal transducer and activator of transcription 6 (STAT6) (a) and the phosphorylated, active form of STAT6 (p-STAT6) (b) in the cell lysates of normal human bronchial epithelial cells incubated with IL-13 (10 ng/mL) or IL-4 (1 ng/mL) for 14 days. Lanes show the data from samples treated with either the vehicle of IL-13 or IL-4 (PBS) (lane 1), IL-13 (10 ng/mL) plus the vehicle of antibody (PBS) (lane 2), IL-13 plus anti-IL-13Ra1 antibody (10 mg/mL) (lane 3), IL-13 plus recombinant human soluble IL-13Ra2/Fc (rsIL-13Ra2, 4 ng/mL) (lane 4), IL-4 (1 ng/mL) plus the vehicle of antibody (PBS) (lane 5) or with IL-4 (1 ng/mL) plus rsIL-13Ra2 (4 ng/mL) (lane 6). The arrows show STAT6 (a) and p-STAT6 (b) (100120 kDa). Data are representative of three differ- ent experiments.

    Journal: Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology

    Article Title: Modulation of mucus production by interleukin-13 receptor alpha2 in the human airway epithelium.

    doi: 10.1111/j.1365-2222.2007.02871.x

    Figure Lengend Snippet: Fig. 10. Western blot analysis of signal transducer and activator of transcription 6 (STAT6) (a) and the phosphorylated, active form of STAT6 (p-STAT6) (b) in the cell lysates of normal human bronchial epithelial cells incubated with IL-13 (10 ng/mL) or IL-4 (1 ng/mL) for 14 days. Lanes show the data from samples treated with either the vehicle of IL-13 or IL-4 (PBS) (lane 1), IL-13 (10 ng/mL) plus the vehicle of antibody (PBS) (lane 2), IL-13 plus anti-IL-13Ra1 antibody (10 mg/mL) (lane 3), IL-13 plus recombinant human soluble IL-13Ra2/Fc (rsIL-13Ra2, 4 ng/mL) (lane 4), IL-4 (1 ng/mL) plus the vehicle of antibody (PBS) (lane 5) or with IL-4 (1 ng/mL) plus rsIL-13Ra2 (4 ng/mL) (lane 6). The arrows show STAT6 (a) and p-STAT6 (b) (100120 kDa). Data are representative of three differ- ent experiments.

    Article Snippet: For STAT6 and the p-STAT6, the membranes were then blocked with 5% skimmed milk in Tris-buffered saline containing 0.1% Tween 20 and incubated with either a monoclonal anti-human/ mouse STAT6 antibody (R&D Systems Inc.) or an affinitypurified rabbit anti-phospho-STAT6 (Y641) antibody (R&D Systems Inc.).

    Techniques: Western Blot, Incubation, Recombinant